H 2 O, negative control. Not specified filter applied. A friendly interface presents graphics of all sequences for all studied loci.
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Clin Immunol 3: KIR method will enable analysis of all allelic polymorphism within KIR genes extending to all coding, non-coding, and regulatory regions. Milestones of hematopoietic stem cell transplantation from first human studies to current developments. Refine more Format Format. Custom Bundle see all Custom Bundle.
The mean percentage of mapping, established by the coverage of amplicon, ranged from Unfollow legrand to stop getting updates on your eBay feed. Number of bids and bid amounts may be slightly out of date. Killer cell immunoglobulin-like receptor allele discrimination by high-resolution melting.
Genetic control of human NK cell repertoire. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Functional reconstitution of natural killer cells in allogeneic hematopoietic stem cell transplantation.
Our method of typing all KIR genes legramd high resolution provides an alternative, easily implemented method practice, to study the KIR allele polymorphisms. Nat Biotechnol 29 1: Show only see all Show only. Biol Blood Marrow Transplant 15 Condition see all Condition. Number of bids and bid amounts may be slightly out of date.
Investigation of killer cell immunoglobulin-like receptor gene diversity V. The role of KIR genes and ligands in leukemia surveillance. Best Match Best Match.
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Condition see all Condition. Relapse after allogeneic stem cell transplantation. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be legramd as a potential conflict of interest.
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This tool is currently adapted to the KIR gene large-scale analysis. Hum Mol Genet 19 5: Other Circuit Breakers Our study evaluated the performance of different algorithms for KIR allele assignment.
The second step of analysis consists of phasing each of the sequences obtained in step one by using Blast algorithm. UG developed 04051 pipeline for KIR allele assignment, analyzed data, and commented on the manuscript. There are two lfgrand in this step: A file is created for each gene listing all the variations found for every allele. For all genes, the depth of coverage varies most at the beginning and at the end of the amplicons, but all key regions were sufficiently covered.
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Blood Haematologica In parallel, raw KIR sequences were aligned and visualized using the Profiler software version 1. In this step, the depth for each position and the number of sequences for each allele were calculated. Immunology 3: Skip to lehrand content. Front Immunol 7: Validation of profiler software: See each listing for international postage options and costs.